Journal: Bioactive Materials

Article Title: Microenvironment-educated MSC-EVs loaded injectable smart hydrogel for targeting senescent nucleus pulposus cells and inhibiting ferroptosis against intervertebral disc degeneration

doi: 10.1016/j.bioactmat.2026.02.030

Figure Lengend Snippet: D-EVs Counteract NPC Senescence by Suppressing Ferroptosis. (A) KEGG pathway analysis of DEGs in senescent NPCs following treatment with D-EVs or not. (B-C) GSEA plots showing significant enrichment of ferroptosis and cell cycle in senescent NPCs. (D-E) Heatmap quantification of key genes involved in ferroptosis and cell cycle. (F) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with different experimental conditions. (G) Representative images of C11-BODIPY 581/591 staining to detect lipid peroxidation (green) in the control, TBHP, Era, Era + Fer-1, or TBHP + Fer-1 groups. (H-I) Quantitative assessment of malondialdehyde (MDA) levels (H) and glutathione (GSH) levels (I) in the control, TBHP, N-EVs, D-EVs, or D-EVs + Era groups. (J) Western blot analysis of key ferroptosis (GPX4, SLC7A11, ACSL4) and senescence (p21, P16) markers in NPCs following treatment with PBS, N-EVs, D-EVs, or D-EVs + Era. (K) Confocal analysis of GPX4 with IF staining in the control, TBHP, N-Evs, D-EVs, and D-EVs + Era group. (L) Flow cytometry analysis of cell cycle distribution in the above experimental conditions. Statistical comparisons were performed between the experimental group and the TBHP-induced group. The data were presented as mean ± SD. n = 3, ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

Article Snippet: After blocked with 5% non-fat milk for 2 h at room temperature, the membranes were incubated with primary antibodies against GAPDH (1:5000, 104941-AP, Proteintech), TSG101 (1:1000, DF8427, Affinity), CD9 (1:1000, AF5139, Affinity), CD63 (1:2000, 25682-1-AP, Proteintech), Calnexin (1:5000, 10427-2-AP, Proteintech), GM130 (1:20000, 11308-1-AP, Proteintech), CXCR3 (1:5000, 26756-1-AP, Proteintech), CXCL10 (1:2000, 10937-1-AP, Proteintech), MMP3 (1:2000, 17873-1-AP, Proteintech), ADAMTS5 (DF13268, Affinity), P16 (AF5484, Affinity), P21 (10355-1-AP, Proteintech), GPX4 (1:1000, 381958, Zen-bio), SLC7A11 (1:1000, 26864-1-AP, Proteintech), ACSL4 (1:5000, 22401-1-AP, Proteintech) and Tubulin (1:10000, T40103 , Abmart) overnight at 4 °C.

Techniques: Western Blot, Staining, Control, Flow Cytometry

Journal: Frontiers in Pharmacology

Article Title: Apocynum venetum L. leaves extract inhibits ferroptosis and improves renal fibrosis in diabetic kidney disease by regulating the Nrf2/HO-1 pathway

doi: 10.3389/fphar.2026.1807373

Figure Lengend Snippet: AVLE inhibits ferroptosis in db/db mice. (A) TEM result. Scale bar = 2 or 0.5 μm, n = 3. (B,C) Identification of GPX4 protein expression in kidney tissues by IHC. Scale bar = 200 μm, n = 3. (D) Ferrous iron levels in kidney tissues of each group, n = 6. (E–G) Western blot for GPX4 and SLC7A11 protein expression with semi-quantitative analysis. β-actin served as the internal control. All data are presented as mean ± SEM (n = 6). Compared with the CON group: # p < 0.05, ## p < 0.01, ### p < 0.001; Compared with the MOD group: * p < 0.05, ** p < 0.01.

Article Snippet: SLC7A11 rabbit monoclonal antibody , 1:1000 , HUABIO , HA721868.

Techniques: Expressing, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Apocynum venetum L. leaves extract inhibits ferroptosis and improves renal fibrosis in diabetic kidney disease by regulating the Nrf2/HO-1 pathway

doi: 10.3389/fphar.2026.1807373

Figure Lengend Snippet: Impact of AVLE on Nrf2, HO-1 protein expression, and ferroptosis in HK-2 cells treated with HG. (A) Each group’s ferrous iron concentration was determined using a kit. (B,C) Each group’s levels of GPX4, SLC7A11, Nrf2, and HO-1 protein expression were identified by Western blot. All data are presented as mean ± SEM (n = 3). Compared with the NG group: # p < 0.05, ## p < 0.01, ### p < 0.001; compared with the HG group: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: SLC7A11 rabbit monoclonal antibody , 1:1000 , HUABIO , HA721868.

Techniques: Expressing, Concentration Assay, Western Blot